Mouse Il1rn (NP_001034790, 27 a.a. - 178 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
20
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 1 EU per 1 ug of protein (determined by LAL method).
Activity:
Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60 ng/mL with Mouse IL-1 alpha.
Quality Control Testing:
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage:
Bioactivity SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl buffer, 0.1 M NaCl, pH 8.0 (10% glycerol).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
Result of bioactivity analysis
Il1rn (Mouse) Recombinant Protein (Cat # P6973) inhibits Mouse IL-1 alpha induced cell proliferation in the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 60 ng/mL.