Mouse Got2 (NP_034455, 30 a.a. - 430 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
46.8
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 90% by SDS-PAGE
Endotoxin Level:
< 1 EU per 1 ug of protein (determined by LAL method).
Activity:
Specific activity is > 20 unit/mg, and is defined as the amount of enzyme that converts 1 umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
Quality Control Testing:
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.