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Last updated: 2023/5/29

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Tnf (Rat) Recombinant Protein BioActive

  • Catalog # : P6958
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  • Specification
  • Product Description:
  • Rat Tnf (NP_038721, 80 a.a. - 235 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
  • Host:
  • Rat
  • Theoretical MW (kDa):
  • 19.9
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 90% by SDS-PAGE
  • Endotoxin Level:
  • > 1 EU per 1ug of protein (determined by LAL method)
  • Activity:
  • Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/ml.
  • Quality Control Testing:
  • 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

    QC Testing of P6958
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Stored at 2°C to 8°C for 1 week.
    For long term storage, aliquot and store at -20°C to -80°C.
    Avoid repeated freezing and thawing cycles.
  • Note:
  • Result of bioactivity analysis

  • Tnf (Rat) Recombinant Protein stimulates cytotoxicity of the L929 mouse fibroblast cells. The ED50 range ≤ 0.2 ng/mL.


  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Tnf
  • Gene Alias:
  • MGC124630,RATTNF,TNF-alpha,Tnfa
  • Gene Description:
  • tumor necrosis factor (TNF superfamily, member 2)
  • Other Designations:
  • tumor necrosis factor superfamily member 2,tumor necrosis factor superfamily, member 2,tumor necrosis factor alpha,tumor necrosis factor superfamily, member 2,tumor necrosis factor, alpha (cachetin),tumor necrosis factor-alpha
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