Human GOT1 (NP_002070, 1 - 413 a.a.) full length recombinant protein with His tag expressed in Escherichia coli expression system
Host:
Escherichia coli
Theoretical MW (kDa):
48.4
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 1 EU/ug
Activity:
Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1 umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl, 100 mM NaCl, 2 mM DTT, pH 8.0 (20% glycerol)
Storage Instruction:
Store at 4°C for one week. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations:
OTTHUMP00000020254,aspartate aminotransferase 1,growth-inhibiting protein 18