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Last updated: 2022/11/27

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GOT2 Human Recombinant Protein BioActive

  • Catalog # : P6933
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  • Specification
  • Product Description:
  • Human GOT2 (NP_002071, 30 - 430 a.a.) partial length recombinant protein with His tag expressed in Escherichia coli expression system
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 47
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 1 EU/ug
  • Activity:
  • Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1 umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6933
    3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl, 0.15 M NaCl, 1 mM DTT, pH 8.0 (10% glycerol)
  • Storage Instruction:
  • Store at 4°C for one week. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 2806
  • Gene Name:
  • GOT2
  • Gene Alias:
  • FLJ40994,KAT4,KATIV,mitAAT
  • Gene Description:
  • glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
  • Gene Summary:
  • Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
  • Other Designations:
  • aspartate aminotransferase 2,kynurenine aminotransferase IV
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