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Last updated: 2022/6/26

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GCK (Human) Recombinant Protein BioActive

  • Catalog # : P6897
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  • Specification
  • Product Description:
  • Human GCK (P35557) recombinant protein expressed in Escherichia coli.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 53.1
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 80% by SDS-PAGE
  • Activity:
  • Specific activity is 303 pmol/min/ug.
    One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 25°C.
    Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2 , 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6897
    GSK (Human) Recombinant Protein (Cat # P6897) was determined by 10% SDS-PAGE with Coomassie Blue, showing a band at 53.1 kDa.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 50 mM potassium phosphate, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, pH 7.4 (2.5% glycerol)
  • Storage Instruction:
  • Store at -80°C for 12 months.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • SDS-PAGE
  • Enzyme Activity
  • Application Image
  • SDS-PAGE
  • Enzyme Activity
  • Gene Information
  • Entrez GeneID:
  • 2645
  • Gene Name:
  • GCK
  • Gene Alias:
  • GK,GLK,HHF3,HK4,HKIV,HXKP,MODY2
  • Gene Description:
  • glucokinase (hexokinase 4)
  • Gene Summary:
  • Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq
  • Other Designations:
  • ATP:D-hexose 6-phosphotransferase,OTTHUMP00000024521,OTTHUMP00000159308,glucokinase,hexokinase D, pancreatic isozyme
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