Escherichia coli Carbonic anhydrase recombinant protein expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
27
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 95% by SDS-PAGE
Activity:
Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37 °C.
Quality Control Testing:
Loading 3 ug protein in 15% SDS-PAGE
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl buffer, pH 8.0 (10% glycerol, 1 mM DTT).
Storage Instruction:
Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.