Human CA8 (NP_004047, 1 a.a. - 290 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
35.5
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 90% by SDS-PAGE
Activity:
Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37°C.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl, 1 mM DTT, pH 8.0 (20% glycerol)
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. [provided by RefSeq
Other Designations:
CA-related protein,carbonate dehydratase,carbonic anhydrase-like sequence,carbonic anhydrase-related protein