Human PGD (NP_002622, 1 a.a. - 483 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
55.3
Form:
Liquid
Preparation Method:
Escherichia coli expression system
Purity:
> 90% by SDS-PAGE
Activity:
Specific activity is > 10 unit/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25°C, in the presence of beta-NADP.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM Tris-HCl, 1 mM DTT, 0.1 M NaCl, pH 8.0 (10% glycerol)
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq