Human SIGLEC15 (NP_998767.1, 20 a.a. - 263 a.a.) partial recombinant protein with Fc, 6x His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat cells. The ED50 for this effect is 1.8-7.2 ug/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
SIGLEC15 (Human) Recombinant Protein (Cat # P6792) was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, 300 mM NaCl, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.