Human NCR3 (NP_001138938.1, 19 a.a. - 138 a.a.) partial recombinant protein with Fc, 6x His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 0.1 EU/ug of the protein by LAL method.
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant Human B7-H6 at 2 ug/mL (100 uL/well) can bind NCR3 (Human) Recombinant Protein (Cat # P6791), the ED50 of Human NCR3 is 66.43 g/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
NCR3 (Human) Recombinant Protein (Cat # P6791) was determined by SDS-PAGE with Coomassie Blue, showing a band at 54-60 kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
Natural cytotoxicity receptors (NCRs), such as NCR3, are activating natural killer (NK) cell receptors that belong to the immunoglobulin (Ig) superfamily. NCR3 is expressed in all resting and activated NK cells and forms a complex with CD3-zeta (CD3Z, or CD247; MIM 186780) (Sato et al., 2001 [PubMed 11782277]).[supplied by OMIM