Human CD276 (Q5ZPR3-2, Leu 29 - Pro 245 ; Pro 99 - Lys 330) partial recombinant protein with Fc, His tag at C-terminal expressed in HEK293 cells.
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% by SDS-PAGE
Activity:
ELISA plate pre-coated by 2 ug/mL (100 uL/well) CD276 (Human) Recombinant Protein (Cat # P6786), mFc-His tagged protein can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
CD276 (Human) Recombinant Protein (Cat # P6786) was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-75 kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4 (5% - 19% Trehalose)
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitute with deionized water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM