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Last updated: 2023/3/19

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LEPR (Human) Recombinant Protein BioActive

  • Catalog # : P6771
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  • Specification
  • Product Description:
  • Human LEPR (NP_002294.2, 20 a.a. - 839 a.a.) partial recombinant protein with Fc and His tag at C-terminus expressed in HEK293 cells.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 170-190
  • Form:
  • Lyophilized
  • Preparation Method:
  • Mammalian cell (HEK293) expression system
  • Purification:
  • Ni-sepharose purification
  • Purity:
  • > 95% by SDS-PAGE
  • Endotoxin Level:
  • < 1.0 EU/ug
  • Activity:
  • Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Leptin protein at 2 ug/mL (100 uL/well) can bind Recombinant Human Leptin R/CD295 protein, the EC50 of Leptin R/CD295 is 261.45 ng/mL.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue

    QC Testing of P6771
    LEPR (Human) Recombinant Protein (Cat #P6771) was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-190 kDa.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store the lyophilized protein at -20°C to -80°C for long term.
    After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Immunogen
  • SDS-PAGE
  • Application Image
  • Immunogen
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3953
  • Gene Name:
  • LEPR
  • Gene Alias:
  • CD295,OBR
  • Gene Description:
  • leptin receptor
  • Gene Summary:
  • Leptin (LEP; MIM 164160), an adipocyte-specific hormone that regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure, acts through the leptin receptor (LEPR), a single-transmembrane-domain receptor of the cytokine receptor family.[supplied by OMIM
  • Other Designations:
  • OB receptor,OTTHUMP00000010693,OTTHUMP00000010694
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