Human CD48 (NP_001769.2, 27 a.a. - 220 a.a.) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Host:
Human
Theoretical MW (kDa):
35-42
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 95% by SDS-PAGE
Endotoxin Level:
< 0.1 EU/ug
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD48 at 5 ug/mL (100 uL/well) can bind recombinant human CD244 with a linear range of 0.2-1 ug/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
CD48 (Human) Recombinant Protein (Cat #P6759) was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-42 kDa.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Aliquot to avoid repeated freezing and thawing.
BLAST1 is the designation used for an activation-associated cell surface glycoprotein of 40 to 45 kD expressed primarily in mitogen-stimulated human lymphocytes. The protein sequence predicted by the cDNA encoding BLAST1 indicates that BLAST1 is a member of the immunoglobulin supergene family. Yokoyama (1991) identified the BLAST1 activation/adhesion molecule as CD48.[supplied by OMIM