Human CD80 (NP_005182.1, 35 a.a. - 242 a.a.) partial recombinant protein with a 6×His tag at the C-terminus expressed in HEK293 cells
Host:
Human
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purification:
Ni-sepharose purification
Purity:
> 92% (determined by SDS-PAGE)
Endotoxin Level:
< 0.1 EU/ug of protein (determined by LAL method)
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD80 at 2 ug/mL (100 uL/well) can bind recombinant human CTLA4 with a linear range of 10-50 ng/mL.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Storage Instruction:
Store at -80°C on dry atmosphere, lyophilized antibodies are stable at least 2 years. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.[supplied by OMIM