S (N501Y) (SARS-CoV-2 Alpha Variant) RBD Recombinant Protein

Product Description:
SARS-CoV-2 Alpha Variant S RBD (QHD43416.1, 319 a.a. - 541 a.a.) N501Y mutant recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.

Activity:
Immobilized S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for human ACE2, Fc tag with the EC50 of 12.7 ng/mL determined by ELISA.

Recommend Usage:
ELISA
SDS-PAGE
Western Blot
The optimal working dilution should be determined by the end user.

Storage Instruction:
Store in working aliquots at -20°C.
After reconstitution, store at -20°C for several months or store at -80°C for a year.
Aliquot to avoid repeated freezing and thawing.

Note:
Result data
A. Immobilized S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for human ACE2, Fc tag with the EC50 of 12.7 ng/mL determined by ELISA.
B. The purity of S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein is greater than 95% as determiend by SEC-HPLC.
C. Human ACE2 captured in protein A chip, can bind S (N501Y) (SARS-CoV-2 Variant B.1.1.7) RBD Recombinant Protein with an affinity constant of 1.74 nM as determined in SPR assap (Biacore T200).