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Last updated: 2023/3/26

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S1 (SARS-CoV-2 Alpha Variant) Recombinant Protein CoronavirusBioActive

  • Catalog # : P6698
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  • Specification
  • Product Description:
  • SARS-CoV-2 Alpha Variant S1 (QIC53204.1, 16 a.a. - 683 a.a.) amino acids 69-70 and 144 deleted; N501Y/A570D/P681H mutant partial recombinant protein with polyHis tag at C-terminal expressed in HEK cells.
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 76
  • Form:
  • Lyophlized
  • Preparation Method:
  • Mammalian cell (HEK) expression system
  • Purification:
  • Affinity chromatography nickle column
  • Purity:
  • greater than 95%.
  • Endotoxin Level:
  • Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1 EU/ug).
  • Activity:
  • Immobilized human ACE2, His Tag at 0.5 ug/mL (100 ul/Well). Dose response curve for this protein with the ED50 of 0.1 ug/mL determined by ELISA.
  • Quality Control Testing:
  • 1 ug loaded Tris-Bis PAGE under reducing condition.

    QC Testing of P6698
    The purity is greater than 95%.
  • Recommend Usage:
  • SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4.
  • Storage Instruction:
  • Store the lyophilized protein at -20°C.
    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffer.
    After reconstitution with distilled water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This molecule has a calculated mass of approximately 76 kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
    Result of activity analysis

  • Applications
  • SDS-PAGE
  • Application Image
  • SDS-PAGE
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