N (SARS-CoV-2) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV NP at 1 ug/mL. Purified recombinant rabbit monoclonal antibodies 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), and 84D7 (Cat # RAB01057) were serially diluted 1:2 starting at 1 ug/mL. Recombinant rabbit monoclonal antibodies 75G5a, 84C4a, and 84D7 show very strong and specific binding to SARS-CoV-2 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
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Specification
Product Description
SARS-CoV-2 N (P0DTC9) full-length recombinant protein with His tag at C-terminal expressed in HEK293 cells.
Host
Human
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-NTA column
Concentration
0.5 mg/mL
Quality Control Testing
SDS-PAGE analysis under reduced and non-reduced conditions.
Recommend Usage
ELISA
The optimal working dilution should be determined by the end user.Storage Buffer
In 1X PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C.
Note
N (SARS-CoV-2) Recombinant Protein (Cat # P6687) is used as control antigen for assays using recombinant rabbit monoclonal antibody clones 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), and 85C1 (Cat # RAB01059) including in biotinylated format clones 75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), and 85C1 (Cat # RAB01060). These antibodies can be paired in sandwich detection assay and used to detect NP antigen from SARS-CoV-2 with high sensitivity (to 2.5 pg) and high specificity.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. -
Applications
Enzyme-linked Immunoabsorbent Assay
Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV NP at 1 ug/mL. Purified recombinant rabbit monoclonal antibodies 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), and 84D7 (Cat # RAB01057) were serially diluted 1:2 starting at 1 ug/mL. Recombinant rabbit monoclonal antibodies 75G5a, 84C4a, and 84D7 show very strong and specific binding to SARS-CoV-2 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
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