AURKB/INCENP (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human AURKB (Q96GD4, 1 a.a. - 344 a.a.) full length recombinant protein with GST tag co-expressed with His-tagged INCENP (AAU04398.1, 803 a.a. - 918 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
Theoretical MW (kDa)
66
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
86 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Kemptide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — INCENP
Entrez GeneID
3619Protein Accession#
Q96GD4Gene Name
INCENP
Gene Alias
FLJ31633, MGC111393
Gene Description
inner centromere protein antigens 135/155kDa
Omim ID
604411Gene Ontology
HyperlinkGene Summary
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM
Other Designations
binds and activates aurora-B and -C in vivo and in vitro|chromosomal passenger protein|inner centromere protein INCENP|inner centromere protein antigens (135kD, 155kD)
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Gene Info — AURKB
Entrez GeneID
9212Protein Accession#
Q96GD4Gene Name
AURKB
Gene Alias
AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, STK12, STK5
Gene Description
aurora kinase B
Omim ID
604970Gene Ontology
HyperlinkGene Summary
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM
Other Designations
aurora-1|aurora-B|serine/threonine kinase 12
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