CSF2 (Human) Recombinant Protein
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Specification
Product Description
Human CSF2 (P04141) recombinant protein expressed in Escherichia coli.
Sequence
MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
Host
Escherichia coli
Theoretical MW (kDa)
14.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliveration of human TF-1 cells. The expected ED50 for this effect is 0.14-0.2 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized from 10 mM Na2PO4, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSF2
Entrez GeneID
1437Protein Accession#
P04141Gene Name
CSF2
Gene Alias
GMCSF, MGC131935, MGC138897
Gene Description
colony stimulating factor 2 (granulocyte-macrophage)
Omim ID
138960Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq
Other Designations
colony stimulating factor 2|granulocyte-macrophage colony stimulating factor|molgramostin|sargramostim
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