MYC (phospho S62) monoclonal antibody, clone 33A12E10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot from crude cell extracts of AGS (gastric adenocarcinoma) cells with MYC (phospho S62) monoclonal antibody, clone 33A12E10 (Cat # MAB6763).
Lane 1 : Scrambled siRNA was introduced into the cells as a negative control.
Lane 2 : Negative control siRNA from Qiagen was transfected.
Lane 3 : siRNA for MYC was transfected.
The data was provided by A. Khanna and Dr. J. Westermark of University of Tampere.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of MYC.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S62 of human MYC.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b, kappa
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot from crude cell extracts of AGS (gastric adenocarcinoma) cells with MYC (phospho S62) monoclonal antibody, clone 33A12E10 (Cat # MAB6763).
Lane 1 : Scrambled siRNA was introduced into the cells as a negative control.
Lane 2 : Negative control siRNA from Qiagen was transfected.
Lane 3 : siRNA for MYC was transfected.
The data was provided by A. Khanna and Dr. J. Westermark of University of Tampere.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYC
Entrez GeneID
4609Gene Name
MYC
Gene Alias
bHLHe39, c-Myc
Gene Description
v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
Other Designations
avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|v-myc avian myelocytomatosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability.
Li J, Chen Y, Qin X, Wen J, Ding H, Xia W, Li S, Su X, Wang W, Li H, Zhao Q, Fang T, Qu L, Shao N.
Nucleic Acids Research 2014 Jan; 42(1):458.
Application：IP, Human, HeLa cells.
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MYC-dependent regulation and prognostic role of CIP2A in gastric cancer.
Khanna A, Bockelman C, Hemmes A, Junttila MR, Wiksten JP, Lundin M, Junnila S, Murphy DJ, Evan GI, Haglund C, Westermarck J, Ristimaki A.
Journal of the National Cancer Institute 2009 May; 101(11):793.
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CIP2A inhibits PP2A in human malignancies.
Junttila MR, Puustinen P, Niemela M, Ahola R, Arnold H, Bottzauw T, Ala-aho R, Nielsen C, Ivaska J, Taya Y, Lu SL, Lin S, Chan EK, Wang XJ, Grenman R, Kast J, Kallunki T, Sears R, Kahari VM, Westermarck J.
Cell 2007 Jul; 130(1):51.
Application：WB-Tr, Human, HEK 293 cells.
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Multiple Ras-dependent phosphorylation pathways regulate Myc protein stability.
Sears R, Nuckolls F, Haura E, Taya Y, Tamai K, Nevins JR.
Genes & Development 2000 Oct; 14(19):2501.
Application：WB-Ce, Rat, REF52 cells.
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MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability.
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