Ptprc monoclonal antibody, clone I3/2.3 (PE/Cy5.5)
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More Files
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Flow Cytometry
BALB/c spleen cells were double-stained with Ptprc monoclonal antibody, clone I3/2.3 (FITC) (Cat # MAB5936) and rat anti-mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against native Ptprc.
Immunogen
Native purified Ptprc from T1M1 (Thy-1-C) cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity Mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms)
Form
Liquid
Conjugation
PE/Cy5.5
Isotype
IgG2b
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were double-stained with Ptprc monoclonal antibody, clone I3/2.3 (FITC) (Cat # MAB5936) and rat anti-mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development.
Trowbridge IS, Thomas ML.
Annual Review of Immunology 1994 Jan; 12:85.
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Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
Johnson P, Greenbaum L, Bottomly K, Trowbridge IS.
The Journal of Experimental Medicine 1989 Mar; 169(3):1179.
Application：Flow Cyt, Mouse, Ψ2 cells .
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Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.
Trowbridge IS.
The Journal of Experimental Medicine 1978 Jul; 148(1):313.
Application：IP, Mouse, Thymocytes, spleen cells and T1M1 cells.
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CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development.
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