IFNG monoclonal antibody, clone B27 (FITC)
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More Files
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Flow Cytometry
Flow Cytometry of PMA and ionomycin stimulated human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-PE followed by intracellular staining with IFNG monoclonal antibody, clone B27 (FITC).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant IFNG.
Immunogen
Recombinant protein corresponding to human IFNG.
Host
Mouse
Reactivity
Human
Specificity
human Interferon-gamma (IFN-gamma)1-2. This is a neutralizing antibody. B27 monoclonal antibody has been used for epitope mapping of human IFN-gamma1. The B27 antibody does not bind to denatured IFN-gamma1.
Form
Liquid
Conjugation
FITC
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (10 uL/106cells): suggested use of the reagent is in a final volume of 100 uL
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Flow Cytometry of PMA and ionomycin stimulated human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-PE followed by intracellular staining with IFNG monoclonal antibody, clone B27 (FITC). -
Gene Info — IFNG
Entrez GeneID
3458Gene Name
IFNG
Gene Alias
IFG, IFI
Gene Description
interferon, gamma
Gene Ontology
HyperlinkGene Summary
Interferon-gamma (IFNG), or type II interferon, is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNG in the immune system stems in part from its ability to inhibit viral replication directly, but most importantly derives from its immunostimulatory and immunomodulatory effects. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 (MIM 186940) and CD8 (see MIM 186910) cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops (Schoenborn and Wilson, 2007 [PubMed 17981204]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE.
Immunological Reviews 1992 Jun; 127:5.
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Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.
Favre C, Wijdenes J, Cabrillat H, Djossou O, Banchereau J, de Vries JE.
Molecular Immunology 1989 Jan; 26(1):17.
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Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
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