BCL2L1 monoclonal antibody, clone 7B2.5 (Biotin)
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More Files
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Flow Cytometry
Murine FL5 cells (FL5-NEO) and FL5 cells transfected with Bcl-xL expression plasmid (FL5-BCL-XL) were fixed with buffered paraformaldehyde and then permeabilized with saponin. The cells were incubated with BCL2L1 monoclonal antibody, clone 7B2.5 followed by phycoerythrin-conjugated goat anti-mouse IgG, and then were analyzed by flow cytometry.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant BCL2L1.
Immunogen
Recombinant protein corresponding to human BCL2L1.
Host
Mouse
Reactivity
Human
Specificity
human Bcl-xL (Mr 29 KDa).
Form
Liquid
Conjugation
Biotin
Isotype
IgG3
Recommend Usage
Flow Cytometry (3 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunoprecipitation
Flow Cytometry
Murine FL5 cells (FL5-NEO) and FL5 cells transfected with Bcl-xL expression plasmid (FL5-BCL-XL) were fixed with buffered paraformaldehyde and then permeabilized with saponin. The cells were incubated with BCL2L1 monoclonal antibody, clone 7B2.5 followed by phycoerythrin-conjugated goat anti-mouse IgG, and then were analyzed by flow cytometry. -
Gene Info — BCL2L1
Entrez GeneID
598Gene Name
BCL2L1
Gene Alias
BCL-XL/S, BCL2L, BCLX, Bcl-X, DKFZp781P2092, bcl-xL, bcl-xS
Gene Description
BCL2-like 1
Omim ID
600039Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq
Other Designations
OTTHUMP00000030550|OTTHUMP00000030551|OTTHUMP00000030553
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Interactome
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Pathway
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Disease
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Publication Reference
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Bax-independent inhibition of apoptosis by Bcl-XL.
Cheng EH, Levine B, Boise LH, Thompson CB, Hardwick JM.
Nature 1996 Feb; 379(6565):554.
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Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL.
Shimizu S, Eguchi Y, Kosaka H, Kamiike W, Matsuda H, Tsujimoto Y.
Nature 1995 Apr; 374(6525):811.
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Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.
Kiefer MC, Brauer MJ, Powers VC, Wu JJ, Umansky SR, Tomei LD, Barr PJ.
Nature 1995 Apr; 374(6524):736.
Application：Flow Cyt, Human, FL5.12, WI-L2 cells.
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Bax-independent inhibition of apoptosis by Bcl-XL.
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