Ptprc exon B-dependent form monoclonal antibody, clone C363.16A (Biotin)
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More Files
- More Functions
Flow Cytometry
BALB/c splenocytes were double-stained with Ptprc exon B-dependent form monoclonal antibody, clone C363.16A (PE) and rat anti-mouse CD8a-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Ptprc exon B-dependent form.
Immunogen
Cloned mouse Th2 cell lines.
Host
Rat
Reactivity
Mouse
Specificity
An exon B-dependent epitope of CD45 glycoprotein.
Form
Liquid
Conjugation
Biotin
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
BALB/c splenocytes were double-stained with Ptprc exon B-dependent form monoclonal antibody, clone C363.16A (PE) and rat anti-mouse CD8a-FITC. Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations.
Hathcock KS, Laszlo G, Dickler HB, Sharrow SO, Johnson P, Trowbridge IS, Hodes RJ.
Journal of Immunology 1992 Jan; 148(1):19.
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A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines.
Bottomly K, Luqman M, Greenbaum L, Carding S, West J, Pasqualini T, Murphy DB.
European Journal of Immunology 1989 Apr; 19(4):617.
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Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
Johnson P, Greenbaum L, Bottomly K, Trowbridge IS.
The Journal of Experimental Medicine 1989 Mar; 169(3):1179.
Application：Flow Cyt, Mouse, Ψ2 cells .
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Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations.
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