Ptprc monoclonal antibody, clone I3/2.3
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More Files
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Flow Cytometry
BALB/c spleen cells were double-stained with Ptprc monoclonal antibody-PE and rat anti-mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Ptprc.
Immunogen
T1M1 (Thy-1-C) cells.
Host
Rat
Reactivity
Mouse
Specificity
Mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms).
Form
Liquid
Isotype
IgG2b
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were double-stained with Ptprc monoclonal antibody-PE and rat anti-mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells.
Haidl ID, Ng DH, Rothenberger S, Johnson P, Jefferies WA.
European Journal of Immunology 1995 Dec; 25(12):3370.
Application：IP, Mouse, Mouse dendritic cells.
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CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development.
Trowbridge IS, Thomas ML.
Annual Review of Immunology 1994 Jan; 12:85.
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Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
Johnson P, Greenbaum L, Bottomly K, Trowbridge IS.
The Journal of Experimental Medicine 1989 Mar; 169(3):1179.
Application：Flow Cyt, Mouse, Ψ2 cells .
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Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells.
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