AOF2 monoclonal antibody, clone 1B2E5
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More Files
- More Functions
Western Blot
Western blot analysis using AOF2 monoclonal antibody, clone 1B2E5 (Cat # MAB5530) against truncated AOF2 recombinant protein (Lane 1), HeLa (Lane 2) and Jurkat (Lane 3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma and kidney carcinoma tissue, showing nuclear localization using AOF2 monoclonal antibody, clone 1B2E5 (Cat # MAB5530) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant AOF2.
Immunogen
Recombinant protein corresponding to human AOF2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:2000)
Immunohistochemistry (Frozen sections) (1:500-1:2000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis using AOF2 monoclonal antibody, clone 1B2E5 (Cat # MAB5530) against truncated AOF2 recombinant protein (Lane 1), HeLa (Lane 2) and Jurkat (Lane 3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma and kidney carcinoma tissue, showing nuclear localization using AOF2 monoclonal antibody, clone 1B2E5 (Cat # MAB5530) with DAB staining.Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AOF2
Entrez GeneID
23028Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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Interactome
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Publication Reference
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Regulation of LSD1 histone demethylase activity by its associated factors.
Shi YJ, Matson C, Lan F, Iwase S, Baba T, Shi Y.
Molecular Cell 2005 Sep; 19(6):857.
Application：WB, Human, HeLa cells.
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LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
Metzger E, Wissmann M, Yin N, Muller JM, Schneider R, Peters AH, Gunther T, Buettner R, Schule R.
Nature 2005 Sep; 437(7057):436.
Application：ChIP, Re-ChIP, IP-WB, IHC-P, IF, Human, Mouse, Testis, Prostate, LNCaP, HeLa cells.
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Regulation of LSD1 histone demethylase activity by its associated factors.
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