CD34 monoclonal antibody, clone 4H11[APG]
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More Files
- More Functions
Flow Cytometry
Surface staining of Kg-1a (human acute myelogenous leukemia cell line) with CD34 monoclonal antibody, clone 4H11[APG] (Cat # MAB3835) - FITC. Total viable cells were used for analysis.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD34.
Immunogen
Native purified CD34 from permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Host
Mouse
Theoretical MW (kDa)
110-115
Reactivity
Human
Specificity
This antibody reacts with Class III epitope on CD34 (Mucosialin), a 110-115 KDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Form
Liquid
Purification
Protein-A affinity chromatography.
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Flow Cytometry
Surface staining of Kg-1a (human acute myelogenous leukemia cell line) with CD34 monoclonal antibody, clone 4H11[APG] (Cat # MAB3835) - FITC. Total viable cells were used for analysis. -
Gene Info — CD34
Entrez GeneID
947Gene Name
CD34
Gene Alias
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Gene Description
CD34 molecule
Omim ID
142230Gene Ontology
HyperlinkGene Summary
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM
Other Designations
CD34 antigen|OTTHUMP00000034733|OTTHUMP00000034734|hematopoietic progenitor cell antigen CD34
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Interactome
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Pathway
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Disease
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Publication Reference
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Hematopoietic stem cell antigen CD34: role in adhesion or homing.
Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM.
Stem Cells and Development 2006 Jun; 15(3):305.
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Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L.
Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM.
Stem Cells and Development 2005 Oct; 14(5):470.
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Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution.
Dao MA, Arevalo J, Nolta JA.
Blood 2002 Jul; 101(1):112.
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Hematopoietic stem cell antigen CD34: role in adhesion or homing.
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