GFAP monoclonal antibody, clone GF-02
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More Files
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Western Blot (Tissue lysate)
Western Blotting analysis (reducing conditions) of GFAP in porcine brain lysate.
Lane 1 : Immunostaining with GFAP monoclonal antibody, clone GF-02 (Cat # MAB3613).
Lane 2 : Immunostaining with Isotype mouse IgM control.
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Specification
Product Description
Mouse monoclonal antibody raised against native GFAP.
Immunogen
Native purified porcine GFAP.
Host
Mouse
Theoretical MW (kDa)
55
Reactivity
Human, Pig
Specificity
This antibody exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 KDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgM
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blotting analysis (reducing conditions) of GFAP in porcine brain lysate.
Lane 1 : Immunostaining with GFAP monoclonal antibody, clone GF-02 (Cat # MAB3613).
Lane 2 : Immunostaining with Isotype mouse IgM control.Immunohistochemistry
Immunocytochemistry
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Gene Info — GFAP
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Publication Reference
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Analysis of glial acidic fibrillary protein in the human entorhinal cortex during aging and in Alzheimer's disease.
Porchet R, Probst A, Bouras C, Draberova E, Draber P, Riederer BM.
Proteomics 2003 Aug; 3(8):1476.
Application：IP, IF, WB-Ti, Human, Frontal cortex, Entorhinal cortex.
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Analysis of glial acidic fibrillary protein in the human entorhinal cortex during aging and in Alzheimer's disease.
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