cAMP monoclonal antibody, clone ABM486
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
ELISA Plot : cAMP immorbolized onto plates, followed by addition of stand cAMP. The cAMP monoclonal antibody, clone ABM486 (Cat # MAB2548) was added subsequentially, and visiualized by chromatogenic substrate. Each samples were triplicated. IC50 was then calculated.
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Specification
Product Description
Mouse monoclonal antibody raised against cAMP.
Immunogen
A chemically linked 3', 5'-Cyclic Adenosine Monophosphate (cAMP).
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes cAMP, not cross react with 5'-AMP, 5'-ADP, 5'-ATP or 3', 5'-cGMP.
Form
Liquid
Purification
Protein A Affinity Purification
Isotype
IgG1
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (Antibody Stabilizer).
Storage Instruction
Store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA Plot : cAMP immorbolized onto plates, followed by addition of stand cAMP. The cAMP monoclonal antibody, clone ABM486 (Cat # MAB2548) was added subsequentially, and visiualized by chromatogenic substrate. Each samples were triplicated. IC50 was then calculated. -
Publication Reference
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α-adrenergic blockade prevented environmental temperature reduction-induced transient portal pressure surge in cirrhotic and portal hypertensive rats.
Hui-Chun Huang, Ching-Chih Chang, Chon Kit Pun, Ming-Hung Tsai, Chiao-Lin Chuang, Shao-Jung Hsu, Yi-Hsiang Huang, Ming-Chih Hou, Fa-Yauh Lee.
Clinical Science 2022 Oct; 136(20):1449.
Application：IF, Rat, Rat femoral artery.
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Role of cAMP inhibition of p44/p42 mitogen-activated protein kinase in potentiation of protein secretion in rat lacrimal gland.
Funaki C, Hodges RR, Dartt DA.
American Journal of Physiology. Cell Physiology 2007 Nov; 293(5):C1551.
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α-adrenergic blockade prevented environmental temperature reduction-induced transient portal pressure surge in cirrhotic and portal hypertensive rats.
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