CASP3 monoclonal antibody, clone CPP32 4-1-18
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CASP3 from HEK293 cell extract using CASP3 monoclonal antibody, clone CPP32 4-1-18 (Cat # MAB2360). Lanes 1 and 2 contain inactive and active CASP3, respectively.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant CASP3.
Immunogen
Recombinant protein corresponding to full length human CASP3.
Host
Mouse
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CASP3 from HEK293 cell extract using CASP3 monoclonal antibody, clone CPP32 4-1-18 (Cat # MAB2360). Lanes 1 and 2 contain inactive and active CASP3, respectively. -
Gene Info — CASP3
Entrez GeneID
836Protein Accession#
P42574Gene Name
CASP3
Gene Alias
CPP32, CPP32B, SCA-1
Gene Description
caspase 3, apoptosis-related cysteine peptidase
Omim ID
600636Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000165054|PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
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Interactome
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Pathway
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Disease
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Publication Reference
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Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition.
Nikiforov MA, Riblett M, Tang WH, Gratchouck V, Zhuang D, Fernandez Y, Verhaegen M, Varambally S, Chinnaiyan AM, Jakubowiak AJ, Soengas MS.
PNAS 2007 Dec; 104(49):19488.
Application：WB-Tr, Human, SK-Mel-103 cells.
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Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c.
Kirsch DG, Doseff A, Chau BN, Lim DS, de Souza-Pinto NC, Hansford R, Kastan MB, Lazebnik YA, Hardwick JM.
The Journal of Biological Chemistry 1999 Jul; 274(30):21155.
Application：WB-Ce, Human, HEK 293 cells.
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Oncogene-dependent apoptosis is mediated by caspase-9.
Fearnhead HO, Rodriguez J, Govek EE, Guo W, Kobayashi R, Hannon G, Lazebnik YA.
PNAS 1998 Nov; 95(23):13664.
Application：WB-Ce, Human, IMR-90-E1A cells.
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Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition.
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