NES monoclonal antibody, clone 10C2
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More Files
- More Functions
Western Blot
Detection of human NES using NES monoclonal antibody, clone 10C2 (Cat # MAB2332). Lane 1 : 5 ug total rat brain protein. Lane 2 : 5 ug human CNS progenitor cell protein.
Immunofluorescence
Visualization of NES in PC-3 cells using NES monoclonal antibody, clone 10C2 (Cat # MAB2332). Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NES.
Immunogen
Recombinant protein corresponding to amino acids 1464-1614 of human NES.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to human nestin.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.09% sodium azide
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Detection of human NES using NES monoclonal antibody, clone 10C2 (Cat # MAB2332). Lane 1 : 5 ug total rat brain protein. Lane 2 : 5 ug human CNS progenitor cell protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
Visualization of NES in PC-3 cells using NES monoclonal antibody, clone 10C2 (Cat # MAB2332). Courtesy of Dolnick Lab, Roswell Park Cancer Institute.Flow Cytometry
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Gene Info — NES
Entrez GeneID
10763Protein Accession#
P48681Gene Name
NES
Gene Alias
FLJ21841, Nbla00170
Gene Description
nestin
Omim ID
600915Gene Ontology
HyperlinkGene Summary
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
Other Designations
OTTHUMP00000032198
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Interactome
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Disease
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Publication Reference
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SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity.
Gangemi RM, Griffero F, Marubbi D, Perera M, Capra MC, Malatesta P, Ravetti GL, Zona GL, Daga A, Corte G.
Stem Cells 2009 Jan; 27(1):40.
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Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody.
Messam CA, Hou J, Major EO.
Experimental Neurology 2000 Feb; 161(2):585.
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SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity.
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