AURKA/AURKB/AURKC (phospho T288/T232/T198) monoclonal antibody, clone RM454
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More Files
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Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysates with AURKA/AURKB/AURKC (phospho T288/T232/T198) monoclonal antibody, clone RM454 (Cat # MAB23238) at a 1:2500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with AURKA/AURKB/AURKC (phospho T288/T232/T198) monoclonal antibody, clone RM454 (Cat # MAB23238) at a 1:50 dilution.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AURKA/AURKB/AURKC.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr288/Thr232/Thr198 of human AURKA/AURKB/AURKC.
Reactivity
Human
Specificity
This antibody reacts to human AURKA/AURKB/AURKC only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. There is no cross-reactivity to AURKA/AURKB/AURKC that is not phosphorylated.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysates with AURKA/AURKB/AURKC (phospho T288/T232/T198) monoclonal antibody, clone RM454 (Cat # MAB23238) at a 1:2500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with AURKA/AURKB/AURKC (phospho T288/T232/T198) monoclonal antibody, clone RM454 (Cat # MAB23238) at a 1:50 dilution. -
Gene Info — AURKA
Entrez GeneID
6790Gene Name
AURKA
Gene Alias
AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7
Gene Description
aurora kinase A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
IPL1-related kinase|OTTHUMP00000031340|OTTHUMP00000031342|OTTHUMP00000031343|OTTHUMP00000031344|OTTHUMP00000031345|OTTHUMP00000166071|aurora-A|aurora-related kinase 1|aurora/IPL1-like kinase|breast-tumor-amplified kinase|serine/threonine kinase 15|serine/
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Gene Info — AURKC
Entrez GeneID
6795Gene Name
AURKC
Gene Alias
AIE2, AIK3, AurC, STK13, aurora-C
Gene Description
aurora kinase C
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
aurora/IPL1-related kinase 3|aurora/IPL1/EG2 protein 2|serine/threonine kinase 13 (aurora/IPL1-like)
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Gene Info — AURKB
Entrez GeneID
9212Gene Name
AURKB
Gene Alias
AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, STK12, STK5
Gene Description
aurora kinase B
Omim ID
604970Gene Ontology
HyperlinkGene Summary
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM
Other Designations
aurora-1|aurora-B|serine/threonine kinase 12
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Interactome
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Disease
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