Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin)
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) binds with Obinutuzumab. Coating antigen: Obinutuzumab, 1 ug/mL. Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) dilution start from 1000 ng/mL. EC50 = 13.09 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
Standard curve of Obinutuzumab Sandwich ELISA. The Obinutuzumab Sandwich ELISA assay is developed by using Anti-Obinutuzumab monoclonal antibody, clone 18H8 (Cat # MAB23131) and Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) as the capture and detection antibodies, respectively. The sensitivity of detecting Obinutuzumab is about 30 pg/mL.
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Specification
Product Description
Mouse monoclonal antibody raised against Obinutuzumab.
Target gene is CD20.Immunogen
Obinutuzumab.
Host
Mouse
Specificity
The product is specific for Obinutuzumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Obinutuzumab monoclonal antibody, clone 18H8 (Cat # MAB23131).
Form
Lyophilized
Conjugation
Biotin
Purification
Protein A purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA (ELISA detection: 0.01-1 ug/mL) (Direct/Indirect ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
Non-inhibitory
Storage Buffer
Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmosphere, lyophilized antibodies are stable at 1 years.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) binds with Obinutuzumab. Coating antigen: Obinutuzumab, 1 ug/mL. Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) dilution start from 1000 ng/mL. EC50 = 13.09 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
Standard curve of Obinutuzumab Sandwich ELISA. The Obinutuzumab Sandwich ELISA assay is developed by using Anti-Obinutuzumab monoclonal antibody, clone 18H8 (Cat # MAB23131) and Anti-Obinutuzumab monoclonal antibody, clone 16B7 (Biotin) (Cat # MAB23133) as the capture and detection antibodies, respectively. The sensitivity of detecting Obinutuzumab is about 30 pg/mL. -
Gene Info — MS4A1
Entrez GeneID
931Gene Name
MS4A1
Gene Alias
B1, Bp35, CD20, LEU-16, MGC3969, MS4A2, S7
Gene Description
membrane-spanning 4-domains, subfamily A, member 1
Omim ID
112210Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq
Other Designations
B-lymphocyte cell-surface antigen B1|CD20 antigen|CD20 receptor
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Interactome
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Pathway
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Disease
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