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Last updated: 2023/5/29

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Anti-Atezolizumab monoclonal antibody, clone 10G9 

  • Catalog # : MAB23119
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Atezolizumab.
    Target gene is PD-L1.
  • Immunogen:
  • Atezolizumab.
  • Host:
  • Mouse
  • Specificity:
  • The product is specific for Atezolizumab. The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) (Cat # MAB23121).
  • Form:
  • Lyophilized
  • Preparation Method:
  • This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Atezolizumab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Blocking
    ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • 0.118 ug/mL
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Anti-Atezolizumab monoclonal antibody, clone 10G9 (Cat # MAB23119) binds with Atezolizumab. Coating antigen: Atezolizumab, 1 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 10G9 dilution start from 1000 ng/mL. EC50 = 13.19 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Sandwich ELISA
  • Sandwich ELISA
  • The Sandwich ELISA assay is developed by using Anti-Atezolizumab monoclonal antibody, clone 10G9 (Cat # MAB23119) and Anti-Atezolizumab monoclonal antibody, clone 6B12 (Biotin) (Cat # MAB23121) as capture and detection antibody, respectively. The sensitivity of detecting Rituximab is about 97 pg/mL.
  • Blocking
  • Blocking
  • Anti-Atezolizumab monoclonal antibody, clone 10G9 (Cat # MAB23119) blocks Atezolizumab binding with PD-L1. Coating antigen: Atezolizumab, 0.5 ug/mL. Anti-Atezolizumab monoclonal antibody, clone 10G9 final concentration: 75 ng/mL. PD-L1 dilution start from 30 ug/mL. IC50= 118.2 ng/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Gene Name:
  • CD274
  • Gene Alias:
  • B7-H,B7H1,MGC142294,MGC142296,PD-L1,PDCD1L1,PDCD1LG1,PDL1
  • Gene Description:
  • CD274 molecule
  • Other Designations:
  • CD274 antigen,OTTHUMP00000021029,programmed cell death 1 ligand 1
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