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Last updated: 2022/6/19

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Anti-Rituximab monoclonal antibody, clone 194D7 (Biotin) 

  • Catalog # : MAB23084
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against Rituximab.
    Target gene is CD20.
  • Immunogen:
  • Rituximab.
  • Host:
  • Rabbit
  • Specificity:
  • The product is specific for Rituximab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody : Anti-Rituximab monoclonal antibody, clone 137C6 (Cat # MAB23082).
  • Form:
  • Lyophilized
  • Conjugation:
  • Biotin
  • Preparation Method:
  • The antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Rituximab.
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (0.005-1 ug/mL (Direct/Indirect))
    Sandwich ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS, pH 7.4 (1% BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
    After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Inhibitory IC50:
  • Non-inhibitory
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Anti-Rituximab monoclonal antibody, clone 194D7 (Biotin) (Cat # MAB23084) binds with Rituximab. Coating antigen: Rituximab, 1 ug/mL. Anti-Rituximab monoclonal antibody, clone 194D7 (Biotin) dilution start from 1000 ng/mL. EC50= 2.889 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
  • Sandwich ELISA
  • Sandwich ELISA
  • The Sandwich ELISA assay is developed by using Anti-Rituximab monoclonal antibody, clone 137C6 (Cat # MAB23082) and Anti-Rituximab monoclonal antibody, clone 194D7 (Biotin) (Cat # MAB23084) as capture and detection antibody, respectively. The sensitivity of detecting Rituximab is about 30 pg/mL.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 931
  • Gene Name:
  • MS4A1
  • Gene Alias:
  • B1,Bp35,CD20,LEU-16,MGC3969,MS4A2,S7
  • Gene Description:
  • membrane-spanning 4-domains, subfamily A, member 1
  • Gene Summary:
  • This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq
  • Other Designations:
  • B-lymphocyte cell-surface antigen B1,CD20 antigen,CD20 receptor
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