SNAP25 monoclonal antibody, clone ABHE-19
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human SNAP25.
Immunogen
A synthetic peptide corresponding to human SNAP25.
Host
Rabbit
Theoretical MW (kDa)
23.315
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of SH-SY5Y cell lysate with SNAP25 monoclonal antibody, clone ABHE-19 (Cat # MAB20724).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — SNAP25
Entrez GeneID
6616Protein Accession#
P60880Gene Name
SNAP25
Gene Alias
FLJ23079, RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2
Gene Description
synaptosomal-associated protein, 25kDa
Omim ID
600322Gene Ontology
HyperlinkGene Summary
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000030267|OTTHUMP00000030268|resistance to inhibitors of cholinesterase 4 homolog|synaptosomal-associated protein 25
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Interactome
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Pathway
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Disease
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