BID monoclonal antibody, clone 4D3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: HeLa cell lysate, Lane 3: A549 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with BID monoclonal antibody, clone 4D3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BID.
Immunogen
Recombinant protein corresponding to amino acids 1-195 of human BID.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: HeLa cell lysate, Lane 3: A549 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with BID monoclonal antibody, clone 4D3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — BID
Entrez GeneID
637Protein Accession#
NP_001187Gene Name
BID
Gene Alias
FP497, MGC15319, MGC42355
Gene Description
BH3 interacting domain death agonist
Omim ID
601997Gene Ontology
HyperlinkGene Summary
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
BH3-interacting domain death agonist|BID isoform ES(1b)|BID isoform L(2)|BID isoform Si6|Human BID coding sequence|OTTHUMP00000196197|apoptic death agonist|desmocollin type 4
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Interactome
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Pathway
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Disease
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Publication Reference
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Posttranslational N-myristoylation of BID as a molecular switch for targeting mitochondria and apoptosis.
Zha J, Weiler S, Oh KJ, Wei MC, Korsmeyer SJ.
Science 2000 Dec; 290(5497):1761.
Application：WB-Ti, Mouse, Liver.
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Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors.
Luo X, Budihardjo I, Zou H, Slaughter C, Wang X.
Cell 1998 Aug; 94(4):481.
Application：WB-Ce, Human, Jurkat cells.
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Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis.
Li H, Zhu H, Xu CJ, Yuan J.
Cell 1998 Aug; 94(4):491.
Application：WB-Ce, Human, Jurkat cells, MCF-7 cells.
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Posttranslational N-myristoylation of BID as a molecular switch for targeting mitochondria and apoptosis.
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