PPM1G monoclonal antibody, clone k1G6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast cancer tissue were incubated with PPM1G monoclonal antibody, clone k1G6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence analysis of 293T cells. The cell was stained with PPM1G monoclonal antibody, clone k1G6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PPM1G.
Immunogen
Recombinant protein corresponding to amino acids 317-546 of human PPM1G.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast cancer tissue were incubated with PPM1G monoclonal antibody, clone k1G6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of 293T cells. The cell was stained with PPM1G monoclonal antibody, clone k1G6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPM1G
Entrez GeneID
5496Protein Accession#
NP_002698Gene Name
PPM1G
Gene Alias
MGC1675, MGC2870, PP2CG, PP2CGAMMA, PPP2CG
Gene Description
protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Omim ID
605119Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
Other Designations
OTTHUMP00000123426|PP2C, gamma|protein phosphatase 1C|protein phosphatase 1G|protein phosphatase 2, catalytic subunit, gamma isoform|protein phosphatase 2C gamma isoform|protein phosphatase magnesium-dependent 1 gamma
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Interactome
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Disease
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Publication Reference
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PP2Cgamma-mediated S-phase accumulation induced by the proteasome-dependent degradation of p21(WAF1/CIP1).
Suh EJ, Kim TY, Kim SH.
FEBS Letters 2006 Nov; 580(26):6100.
Application：WB, Human, HEK 293 cells.
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Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3.
Flajolet M, Rakhilin S, Wang H, Starkova N, Nuangchamnong N, Nairn AC, Greengard P.
PNAS 2003 Dec; 100(26):16006.
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PP2Cgamma-mediated S-phase accumulation induced by the proteasome-dependent degradation of p21(WAF1/CIP1).
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