PRKAA2 (phospho S345) monoclonal antibody, clone HFA-16

Product Description:
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PRKAA2.

Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding S345 of human PRKAA2.

Recommend Usage:
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.

Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).

Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Western Blot (Cell lysate)
Western blot analysis of PRKAA2 (phospho S345) expression in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.

Gene Summary:
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq

Other Designations:
5'-AMP-activated protein kinase, catalytic alpha-2 chain,AMP-activated protein kinase alpha 2 catalytic subunit,AMPK-alpha-2 chain,OTTHUMP00000009993