Rabbit monoclonal antibody raised against synthetic peptide of Firefly Luciferase .
Immunogen:
A synthetic peptide corresponding to amino acids 171-190 of Firefly Luciferase .
Sequence:
HLPPGFNEYDFVPESFDRDK
Host:
Rabbit
Theoretical MW (kDa):
60.745
Reactivity:
Firefly
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Western Blot (1:1000-1:5000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Lane 1: Firefly Luciferase transfected 293T, Lane 2: HeLa, Lane 3: NIH/3T3 and Lane 4: C6 cell lysates with Firefly Luciferase monoclonal antibody, clone DAB-6 (Cat # MAB20046).