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ESR1 monoclonal antibody, clone IE-5 

  • Catalog # : MAB20014
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human ESR1.
  • Immunogen:
  • A synthetic peptide corresponding to human ESR1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 66.216
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP (1:30)
    Flow Cytometry (1:30)
    Immunocytochemistry (1:50-1:100)
    Immunofluorescence (1:50-1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
    Immunoprecipitation
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: MCF7 and Lane 2: T47-D cell lysates with ESR1 monoclonal antibody, clone IE-5 (Cat # MAB20014).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cervix carcinoma with ESR1 monoclonal antibody, clone IE-5 (Cat # MAB20014).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 2099
  • Gene Name:
  • ESR1
  • Gene Alias:
  • DKFZp686N23123,ER,ESR,ESRA,Era,NR3A1
  • Gene Description:
  • estrogen receptor 1
  • Gene Summary:
  • This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000017718,OTTHUMP00000017719,estrogen receptor alpha
  • Related Disease
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