DNMT3A monoclonal antibody, clone EOI-4
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human DNMT3A.
Immunogen
A synthetic peptide corresponding to human DNMT3A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:100-1:500)
Immunofluorescence (1:100-1:500)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with DNMT3A monoclonal antibody.Immunocytochemistry
Immunofluorescence
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Gene Info — DNMT3A
Entrez GeneID
1788Protein Accession#
Q9Y6K1Gene Name
DNMT3A
Gene Alias
DNMT3A2, M.HsaIIIA
Gene Description
DNA (cytosine-5-)-methyltransferase 3 alpha
Omim ID
602769Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DNA MTase HsaIIIA|DNA cytosine methyltransferase 3 alpha|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|OTTHUMP00000120005|OTTHUMP00000120006
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