KRT14 monoclonal antibody, clone FEF-11
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human KRT14.
Immunogen
A synthetic peptide corresponding to human KRT14.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A431 cell lysate with KRT14 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human stomach with KRT14 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HaCaT cells with KRT14 monoclonal antibody. -
Gene Info — KRT14
Entrez GeneID
3861Protein Accession#
P02533Gene Name
KRT14
Gene Alias
CK14, EBS3, EBS4, K14, NFJ
Gene Description
keratin 14
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq
Other Designations
cytokeratin 14|keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
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Interactome
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Disease
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