PRKAG1 monoclonal antibody, clone GDE-16
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HEK293 cell lysate with PRKAG1 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human PRKAG1.
Immunogen
A synthetic peptide corresponding to human PRKAG1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Flow Cytometry (1:20)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HEK293 cell lysate with PRKAG1 monoclonal antibody.Immunoprecipitation
Flow Cytometry
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Gene Info — PRKAG1
Entrez GeneID
5571Protein Accession#
P54619Gene Name
PRKAG1
Gene Alias
AMPKG, MGC8666
Gene Description
protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Omim ID
602742Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, gamma-1 subunit|AMP-activated protein kinase, noncatalytic gamma-1 subunit|AMPK gamma-1 chain
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Interactome
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Pathway
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Disease
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