ACTA2 monoclonal antibody, clone EO-1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1)A549 cell lysate; (2)C2C12 cell lysate using alpha-SMA antibody with ACTA2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human colon with ACTA2 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ACTA2.
Immunogen
A synthetic peptide corresponding to human ACTA2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Flow Cytometry (1:20)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1)A549 cell lysate; (2)C2C12 cell lysate using alpha-SMA antibody with ACTA2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human colon with ACTA2 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — ACTA2
Entrez GeneID
59Protein Accession#
P62736Gene Name
ACTA2
Gene Alias
AAT6, ACTSA
Gene Description
actin, alpha 2, smooth muscle, aorta
Omim ID
102620Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020042|alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
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Interactome
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Pathway
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Disease
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