ALAS1 monoclonal antibody, clone EOA-1
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ALAS1.
Immunogen
A synthetic peptide corresponding to human ALAS1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 cell lysate with ALAS1 monoclonal antibody.Immunocytochemistry
Immunofluorescence
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Gene Info — ALAS1
Entrez GeneID
211Protein Accession#
P13196Gene Name
ALAS1
Gene Alias
ALAS, ALAS3, ALASH, MIG4
Gene Description
aminolevulinate, delta-, synthase 1
Omim ID
125290Gene Ontology
HyperlinkGene Summary
Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM
Other Designations
aminolevulinate, delta, synthase 1|migration-inducing protein 4
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