ACLY monoclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of ACLY monoclonal antibody (Cat # MAB11167) at 1:1000 dilution.
Lane 1: K-562 whole cell lysate 40 ug/lane.
Lane 2: Jurkat whole cell lysate 40 ug/lane.
Lane 3: HeLa whole cell lysate 40 ug/lane.
Predicted band size: 120 KDa. Observed band size: 120 KDa
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant protein of ACLY.
Immunogen
Recombinant protein corresponding to C-terminus of human ACLY.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ACLY monoclonal antibody (Cat # MAB11167) at 1:1000 dilution.
Lane 1: K-562 whole cell lysate 40 ug/lane.
Lane 2: Jurkat whole cell lysate 40 ug/lane.
Lane 3: HeLa whole cell lysate 40 ug/lane.
Predicted band size: 120 KDa. Observed band size: 120 KDaEnzyme-linked Immunoabsorbent Assay
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Gene Info — ACLY
Entrez GeneID
47Protein Accession#
NM_001096.2Gene Name
ACLY
Gene Alias
ACL, ATPCL, CLATP
Gene Description
ATP citrate lyase
Omim ID
108728Gene Ontology
HyperlinkGene Summary
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164773
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
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