KLF4 monoclonal antibody, clone 1E6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using KLF4 monoclonal antibody, clone 1E6 (Cat # MAB10714) against HEK293 (1) and KLF4-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (upper) and lung cancer tissues (bottom) using KLF4 monoclonal antibody, clone 1E6 (Cat # MAB10714) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of Eca-109 cells using KLF4 monoclonal antibody, clone 1E6 (Cat # MAB10714) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KLF4.
Immunogen
Recombinant protein corresponding to human KLF4.
Host
Mouse
Theoretical MW (kDa)
54
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using KLF4 monoclonal antibody, clone 1E6 (Cat # MAB10714) against HEK293 (1) and KLF4-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (upper) and lung cancer tissues (bottom) using KLF4 monoclonal antibody, clone 1E6 (Cat # MAB10714) with DAB staining.Immunofluorescence
Immunofluorescence analysis of Eca-109 cells using KLF4 monoclonal antibody, clone 1E6 (Cat # MAB10714) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KLF4
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Interactome
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Disease
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