HSPD1 monoclonal antibody, clone 3G8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) against T-47D (1) , HeLa (2) , HepG2 (3) , A-549 (4) , Jurkat (5) , HEK293 (6) , NIH/3T3 (7) , PC-12 (8) and COS-7 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (A) , kidney cancer tissues (B) , breast cancer tissues (C) and colon cancer tissues (D) using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) .
Flow Cytometry
Flow cytometric analysis of HeLa cells using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HSPD1.
Immunogen
Recombinant protein corresponding to human HSPD1.
Host
Mouse
Theoretical MW (kDa)
61
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) against T-47D (1) , HeLa (2) , HepG2 (3) , A-549 (4) , Jurkat (5) , HEK293 (6) , NIH/3T3 (7) , PC-12 (8) and COS-7 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (A) , kidney cancer tissues (B) , breast cancer tissues (C) and colon cancer tissues (D) using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) with DAB staining.Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) .Flow Cytometry
Flow cytometric analysis of HeLa cells using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) (green) and negative control (purple). -
Gene Info — HSPD1
Entrez GeneID
3329Gene Name
HSPD1
Gene Alias
CPN60, GROEL, HLD4, HSP60, HSP65, HuCHA60, SPG13
Gene Description
heat shock 60kDa protein 1 (chaperonin)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq
Other Designations
P60 lymphocyte protein|chaperonin|heat shock 60kD protein 1 (chaperonin)|heat shock protein 65|mitochondrial heat shock 60kD protein 1 variant 1|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1|spastic paraplegia 13 (autosomal dominant)
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Interactome
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Pathway
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Disease
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